second etbf strain Search Results


second etbf strain  (ATCC)
99
ATCC second etbf strain
bfpai locus deletion impairs fitness during competitive secondary colonization. (A-B) Mice were primarily colonized with NTBF strain TM4000 638R and subsequently challenged with either <t>ETBF</t> <t>strain</t> <t>ATCC</t> 43859 or an isogenic mutant with bfpai deleted (Δbfpai). Clindamycin (100 mg/L) was maintained in drinking water throughout the experiment. Fecal CFU was monitored for 5 weeks post-challenge. N = 4 mice per group. Figures are representative of 3 independent experiments. Error bars: mean +/− s.e.m (A), mean +/− s.d. (B). Dashed lines indicate the limit of detection. n.s., not significant.
Second Etbf Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bfpai locus deletion impairs fitness during competitive secondary colonization. (A-B) Mice were primarily colonized with NTBF strain TM4000 638R and subsequently challenged with either ETBF strain ATCC 43859 or an isogenic mutant with bfpai deleted (Δbfpai). Clindamycin (100 mg/L) was maintained in drinking water throughout the experiment. Fecal CFU was monitored for 5 weeks post-challenge. N = 4 mice per group. Figures are representative of 3 independent experiments. Error bars: mean +/− s.e.m (A), mean +/− s.d. (B). Dashed lines indicate the limit of detection. n.s., not significant.

Journal: Gut Microbes

Article Title: The Bacteroides fragilis pathogenicity island links virulence and strain competition

doi: 10.1080/19490976.2017.1290758

Figure Lengend Snippet: bfpai locus deletion impairs fitness during competitive secondary colonization. (A-B) Mice were primarily colonized with NTBF strain TM4000 638R and subsequently challenged with either ETBF strain ATCC 43859 or an isogenic mutant with bfpai deleted (Δbfpai). Clindamycin (100 mg/L) was maintained in drinking water throughout the experiment. Fecal CFU was monitored for 5 weeks post-challenge. N = 4 mice per group. Figures are representative of 3 independent experiments. Error bars: mean +/− s.e.m (A), mean +/− s.d. (B). Dashed lines indicate the limit of detection. n.s., not significant.

Article Snippet: Moreover, a second ETBF strain (ATCC 43858) is prevented from invading the microbiota of mice colonized with TM4000 despite an intact bfpai .

Techniques: Mutagenesis

Complex protein-level regulation of BFT by environmental cues. (A) Bacteria from stationary phase cultures of ETBF strain ATCC4 3858 were sedimented, resuspended in spent media from normal growth conditions (UT) or fresh BHIS at pH 5.6 and pH 7.4, incubated for 1 hour at 37°C, and separated into pellet and supernatant fractions. BFT in the supernatant fraction was analyzed by Western blot for full length protoxin (FLBFT, upper band) and cleaved active toxin (BFT*). (B) Heat and oxidative stress upregulate BFT production. ETBF strain ATCC 43858 was grown to late-log phase, a growth phase during which BFT expression is not normally detected. Cultures were then exposed to different conditions for 1 hour: normal growth conditions (UT), 42°C, room temperature (RT), or room air (O2). Cell pellet and supernatant fractions were probed for full length protoxin (FLBFT, upper band) and cleaved active toxin (BFT*). The lower band is nonspecific.

Journal: Gut Microbes

Article Title: The Bacteroides fragilis pathogenicity island links virulence and strain competition

doi: 10.1080/19490976.2017.1290758

Figure Lengend Snippet: Complex protein-level regulation of BFT by environmental cues. (A) Bacteria from stationary phase cultures of ETBF strain ATCC4 3858 were sedimented, resuspended in spent media from normal growth conditions (UT) or fresh BHIS at pH 5.6 and pH 7.4, incubated for 1 hour at 37°C, and separated into pellet and supernatant fractions. BFT in the supernatant fraction was analyzed by Western blot for full length protoxin (FLBFT, upper band) and cleaved active toxin (BFT*). (B) Heat and oxidative stress upregulate BFT production. ETBF strain ATCC 43858 was grown to late-log phase, a growth phase during which BFT expression is not normally detected. Cultures were then exposed to different conditions for 1 hour: normal growth conditions (UT), 42°C, room temperature (RT), or room air (O2). Cell pellet and supernatant fractions were probed for full length protoxin (FLBFT, upper band) and cleaved active toxin (BFT*). The lower band is nonspecific.

Article Snippet: Moreover, a second ETBF strain (ATCC 43858) is prevented from invading the microbiota of mice colonized with TM4000 despite an intact bfpai .

Techniques: Bacteria, Incubation, Western Blot, Expressing